Banana peels (BPs) have an excellent nutrient content due to presence of many bioactive compounds. However, an enormous amount of them is wasted daily, leading to environmental pollution. This study aimed to evaluate the anti-microbial and in vitro cytotoxic activities of green and ripe peel extracts (GPE and RPE, respectively) of banana. Total phenolic (TPC), total flavonoid (TFC), saponin contents, DPPH, and total antioxidant capacity (TAC), total protein (T.P), lipid (T.L), carbohydrate (T.C), were detected in GPE and RPE. Gas-chromatography mass spectrophotometry (GC-MS) analysis was determined in BPs extracts. Anti-fungal, anti-bacterial and cytotoxic activities were determined. The results showed that the TPC, TFC, saponin, T.P, T.L T.C and TAC contents in GPE were much higher than those in RPE. According to the results, only hexadecanoic acid was found in both extracts. Both GPE and RPE did not show anti-fungal activity, however, the GPE showed superior anti-bacterial and cytotoxic activities than RPE. Overall, the GPE had higher potential anti-microbial and cytotoxic effects than RPE