This investigation was conducted for two consecutive seasons (2021 & 2022) in El-Baramon farm located in Mansoura City, Dakahlia Governorate, Egypt, to study influence of chitosan and calcium gluconate on growth, yield and fruit quality as well as storability of Crimson Seedless grape cultivar. Seven-year-old vines were cultivated 2*3 meters apart in clay soil with flood irrigation and trellised using the Spanish baron system. In the final week of February, vines were cane-pruned and bud loaded with 96 buds per vine. Nine treatments were conducted as follows: foliar spraying with chitosan at the two concentrations (0.5 or 1%) were applied either separately or in combination with calcium gluconate at the two concentrations (1% or 2%), beside to the control treatment. All different concentrations of chitosan and calcium gluconate were foliar sprayed twice: after fruit set stage when the berry diameter reaches 2-3 mm and at veraison stage (20% berry color). Clusters of all treatments were stored for 4 weeks at ± 0oC and 90 - 95% RH. The combined application of 1% chitosan and 2% calcium gluconate exhibited the most favorable results in terms of acquiring the best aspects of vegetative growth, increasing yield and enhancing cluster quality attributes as well as improving the storability of Crimson Seedless grape via reducing physiological loss in weight, decay and shattering, maintaining firmness and delaying the changes in total soluble solids, acidity and total anthocyanin.