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310903

MOLECULAR CHARACTERIZATION OF RESISTANCE TO EXTENDEDSPECTRUM β-LACTAMS IN KLEBSIELLA PNEUMONIAE ANDKLEBSIELLA OXYTOCA ISOLATES FROM MEAT AND MEAT PRODUCTS

Article

Last updated: 29 Dec 2024

Subjects

-

Tags

Veterinary microbiology, nanotechnology and immunology.

Abstract

Prevalence of K. pneumoniae and K. oxytoca in meat and meat products was estimated in the present study. Four-hundred seventy samples of meat and meat products. (40 imported frozen minced meat,35 imported frozen meat, 25 local meat, 24 Local minced meat, 34 kofta, 46 sausage, 37 hot dog, 29 canned beef, 106 luncheon, 43 basterma, 51 beef burger) were
collected randomly from different retail shops. The prevalence of K. oxytoca was higher in meat (9.7%) than in meat products (7.9%), while the rate of isolation of K. pneumoniae was the same in meat and meat products (11.3%). The isolation rate was higher in imported minced meat (10% for K. oxytoca and 15% for K. pneumoniae) in comparison with the local minced meat (K. oxytoca 8.3% and K.pneumoniae12.5%). All samples of canned beef were negative. The highest isolation rate among the meat product samples was from luncheon (11.3% K. oxytoca and 16.0% K. pneumoniae) and basterma (9.3% K. oxytoca and13.9% K.[pneumoniae) and the lowest was in beef burger (3.9% K. oxytoca and7.8% K. pneumoniae). Twelve K. pneumoniae and K. oxytoca isolates were investigated for antimicrobial resistance against β-lactams groups of antibiotics. The resistance of the isolates to cephalothin was 100%, ampicillin 91.7%, cefpodoxime 75%, cefotaxime 66.7%,
sulfamethazole 41.7%, ceftazidime 33.3%, ceftriaxone16.7%, imipenem and cefepime 8.3%. The 12 isolates of Klebsiellae (5K. oxytoca and 7 K. pneumoniae) were tested for PEH gene (gene of identification of K. oxytoca) and β-lactam resistance genes (shv, tem, ctx-m). The shv gene was detected in 12 (100%) of K. pneumoniae and K. oxytoca isolates, tem gene
was detected in 11 (91.7%) isolates (5 isolates of K. oxytoca and 6 isolates of K. pnemoniae); while ctx-m gene was detected in 9(75%) isolates (4 isolates of K. oxytoca and 6 isolates of K. pnemoniae).

Keywords

Klebsiella pneumoniae, Klebsiella oxytoca, Imported frozen meat, local meat, meat products, extended spectrum beta-lactamase, antimicrobial resistances

Volume

76

Article Issue

1

Related Issue

42738

Issue Date

2016-04-01

Receive Date

2023-08-02

Publish Date

2016-04-01

Page Start

31

Page End

47

Print ISSN

1110-1288

Link

https://jevma.journals.ekb.eg/article_310903.html

Detail API

https://jevma.journals.ekb.eg/service?article_code=310903

Order

310,903

Type

Original Research Articles

Type Code

2,724

Publication Type

Journal

Publication Title

Journal of the Egyptian Veterinary Medical Association

Publication Link

https://jevma.journals.ekb.eg/

MainTitle

MOLECULAR CHARACTERIZATION OF RESISTANCE TO EXTENDEDSPECTRUM β-LACTAMS IN KLEBSIELLA PNEUMONIAE ANDKLEBSIELLA OXYTOCA ISOLATES FROM MEAT AND MEAT PRODUCTS

Details

Type

Article

Created At

18 Dec 2024