Lactic Acid Bacteria (LAB) is a digestive tract microflora that have a beneficial role in fowl energy. The number and difference of LAB in the digestive tract are damaged by various determinants. The objective of this study to reach out isolate, identify, preserve and decide the quantitative level of the Lactobacillus strains from the gut content of 18-day-old chickens'
broilers. Lactobacillus strains were phenotypically recognized and preserved from the gut content of 50 chickens' broilers. Identification was accomplished by morphological, cultural and biochemical characters. The all-inclusive level of Lactobacillus strains in stomach content (105 - 109 CFU/g). According to sequencing of the 16S ribosomal RNA gene fragment, strains of L. crispatus from the gut content of chickens were unique, phenotypically recognized, and which augments their request as a probiotic supplement. Conclusively, L. crispatus strains weretechnologically and ecologically appropriate probiotic.