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309136

IMMUNOHISTOCHEMICAL STUDIES ON METRITIS IN SHE-CAMELS

Article

Last updated: 29 Dec 2024

Subjects

-

Tags

Theriogenology, breeding, artificial insemination and dairy science.

Abstract

The reproductive efficiency of dromedary camels is very low and it is a major problem in camelids. So, the aim of this study was to evaluate the uterine infection of slaughtered she-camels through identification of bacterial agents colonizing the uterine environment, with observing the uterine histopathological picture, immunohistochemical and immunological
view characterization of endometritis in order to find out which factors possibly influence the progress of endometritis. A total of 100 uteri from slaughtered adult she -camels were collected for this study from Kerdassa, Nahia and El-Mounieb abattoirs. S. aureus, E. coli, S. pyogen and P. multocida were isolated in 38 samples as single or mixed infections with
percentage (52.5%) and (47.5%) respectively. S. aureus was the most isolated bacteria as single infection with percentage of 26.2% followed by E. coli 15.8 % then S. pyogen (10.5%) while in mixed infection S. aureus + E.coli were the most prevalent mixed infection as they represented 15.8% followed by S. aureus + S. pyogen with a percentage of 10.5%. According to the histopathological examination, the affections were classified into chronic endometritis was the most common recorded endometritis type (68.4 %) followed by acute endometritis (23.7 %) then sub-acute endometritis (7.9 %). The uterus showed degenerative changes in the uterine glands with marked inflammatory cell infiltrations and edema besides congestion and vasculitis. In addition to, connective tissue proliferation and hyperplasia of endometrial mucosa were seen in chronic cases. Moreover, demonstration of immune cells in chronic endometrial speciments by immunohistochemistry revealed the distribution and type of immune cell as increasing in CD3, CD20 and CD68 with the severity of inflammation associated with the presence of CD138. By measuring the levels of some selected immunological parameters in the blood serum samples as biomarkers for the immune status of both she-camels with sub-acute, acute endometritis NO, Hp and SAA concentrations were significantly higher in serum samples of she-camels with acute endometritis than that of sub-acute endometritis, and in the sub-acute cases when compared with the samples of
apparently normal she-camels. The dendrogram analysis illustrated that there were weak similarities in the protein fingerprints between samples from she-camels infected with subacute endometritis and that of apparently normal she-camels ranging from (0.25 - 0.33).


Keywords

She, camels, Bacteria, histopathology, Immunohistochemistry, Acute phase protein, lysozyme

Volume

77

Article Issue

2

Related Issue

42530

Issue Date

2017-07-01

Receive Date

2023-07-20

Publish Date

2017-07-01

Page Start

379

Page End

406

Print ISSN

1110-1288

Link

https://jevma.journals.ekb.eg/article_309136.html

Detail API

https://jevma.journals.ekb.eg/service?article_code=309136

Order

309,136

Type

Original Research Articles

Type Code

2,724

Publication Type

Journal

Publication Title

Journal of the Egyptian Veterinary Medical Association

Publication Link

https://jevma.journals.ekb.eg/

MainTitle

IMMUNOHISTOCHEMICAL STUDIES ON METRITIS IN SHE-CAMELS

Details

Type

Article

Created At

18 Dec 2024