Fishes are generally regarded as safe, nutritious and beneficial but, aquaculture products have sometimes been associated with certain food safety hazards and the majority of which are usually related to exposed stress. In Egypt, tilapias aquaculture yield represents the most popular cultured species are mainly Oreochromis niloticus. The current study was conducted on tilapias farms at Damietta governorate. Therefore, a total of 50 tilapias fish samples (25 sample from a risky southern area and 25 samples from a non-risky northern area) were random collected from farms in the two local areas for bacterial and chemical risks evaluation. Where, the obtained results showed that incidence of coliforms contamination were 100% and 84% in risky and non-risky area respectively. While, by using conventional culture method a different pathogenic bacterial species were isolated and identified in the flesh muscles with isolation rates 44%, 40% and 60% in risky area. Whereas, the rates were 16%, 8% and 24% in non-risky area for pathogenic E. coli, L. monocytogens and Staph. aureus respectively. Salmonella spp. failed to be isolated from the two areas. Isolates of pathogenic E. coli were subjected to serological identification as O86a, O146, O114, O142 and untypable serovars. The study was conducted to analyze some organochlorine pesticides residues by Agilent gas chromatograph GC. The results revealed rates of 100% heptachlor, 40% dieldrin, 48% DDD, 64% DDT, 16% gamma BHC and 20% delta BHC. Contamination of fish in risky area was exceeding the permissible limits, comparing to the non-risky area where the same residues are detected by lower ratios and within the permissible limits, except for DDD and DDT that was higher than the permissible limit. Organophosphorus pesticides were not detected in all examined samples in the two areas. Cadmium residues were detected in all examined fish flesh samples within the permissible limits, while lead and mercury heavy metal residues were not detected in both risky and non-risky study areas. The public health significance of isolates and chemical pollutants was described