A total of 494 staphylococci isolates, obtained from fifty raw milk samples. Identification of
these isolates was carried out using combined phenotypic and molecular methods. Only 188
isolates were coagulase positive from 44 positive samples that confirmed using Latex Slide Agglutination Test, Staphytect plus. One hundred and fifty eight isolates were Staphylococcus aureus identified using Mannitol Salt Agar Test. Only 117/158 isolates were positive encoding genes for S. aureus using multiplex PCR. Selection of random isolates from different raw milk samples were examined for Staphylococcal Enterotoxin production and the percentage of positive isolates for toxin production was (8.1) of SEE toxin and (2.7) of SEC toxin. The sensitivity and specificity of the tube coagulase test was markedly improved when TNase was introduced as a combination test for routine identification of Staphylococcus aureus (100% specificity). The investigation highlighted that S. aureus is very common and constitute a potential risk for consumers' health.