Aflatoxins are cancerogenic compounds produced predominantly by certain strains of the Aspergillus spp., Aflatoxin M1 (AFM1) is the major hydroxylation of aflatoxin B1 (AFB1), which is formed when animals ingest feed contaminated with aflatoxin B1. Contamination of milk and dairy products with aflatoxin M1 is a risk for human health. Afloatoxin M1 is relatively stable during milk pasteurization and storage as well as during the preparation of various dairy products. In this study, 80 samples represented by 20 samples of animal feed, 20 of raw milk, 20 of cream and 20 of butter were obtained from suppliers of the dairy company under study. The concentration of aflatoxin B1 and M1 were determined by high pressure liquid chromatography (HPLC). Presence of AFB1 was detected in animal feed by a ratio of 55% in concentrations ranged between 7.16-69.81ppb with a mean value of 32.06 ± 1.47 ppb. While mean value of AFM1 in samples of raw milk, cream and butter (20 of each), were 30%, 25% and 25% with a mean value of 0.429 ± 0.031 , 0.272 ± 0.015 and 0.053 ± 0.003 ppb., respectively, while the acceptability of positive samples were 70%,75%, and 90%, respectively according to EOS, EC and Codex Regulation.