Crustaceans and Molluscus, as Crab and Oyster are filter-feeding organisms that concentrate microorganisms, especially the human enteric viruses and pathogenic Vibrio species. This study aimed to detect and accurate identify Vibrio sp. in fresh and marine Crab and Oyster specimens especially that of public health important as cholerae, Vibrio parahaemolyticus, Vibrio mimicus and Vibrio vulnificususing using biochemical methods and PCR methods (16SrRNA and mPCR). The prevalence of Vibrio species was 5%, 50% and 65% in fresh Crab, marine Crab and Oyster samples respectively examined by traditional biochemical method appeared in examined fresh Crab samples as only one vibrio mimicus species as (5%). Vibrio species in collected marine Crab samples was V. alginolyticus (15%), V. cholera (5%), V. parahaemolyticus (10%) and V. vulnificus (20%). Vibrio species in oyster was V. alginolyticus (5%), V. cholera ( 10%), V. mimicus (5%), V. parahaemolyticus (20%) and V.vulnificus (25%). Overall, results of the present study indicated that the using of 16SrRNA and mPCR allowed rapid and accurate identification of the predominant and pathogenic Vibrio species which failed to be accurately identified by biochemical method. Furthermore, presence of Vibrio cholerae and Vibrio mimicus in examined samples considered a life threatening to human health.