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248064

Validation of HPLC Method for Determination of Doxycycline hyclate in turkey serum

Article

Last updated: 29 Dec 2024

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Tags

Chemical Contaminants of food of animal origin

Abstract

An accurate, sensitive and reproducible high performance liquid chromatographic (HPLC) method for the quantitation of doxycycline in turkey serum has beendeveloped and validated. The doxycycline standard was eluted from a C18 column (4.6 mm i.d., 250 mm, 5 µm particle size) at 15 ⁰C with a mobile phase consisting of 5% acetic acid : methanol : acetonitrile (55:20:25, v/v/v). A UV detector set at 347 nm was used to monitor the effluent. Retention time of doxycycline was approximately at 4 min and the run time was 10 min. The column temperature adjusted at 15 ⁰C with a flow rate 1ml/min and injection volume 25 µl. Doxycycline was extracted from serum by ACN. Linearity was confirmed in the concentration range 0.1-2 µg/ml, had regression coefficients (R2) 0.9999 for doxycycline quantification in turkey serum. The analytical conditions gave a recoveries in a range of 97.57% to 99.83%. The limit of detection (LOD) was 0.035 µg/ mL and limit of quantification (LOQ) was 0.108 µg/ml. The relative standard deviation (RSD %) was less than 2% in all cases. The developed method was accurate, precise and rapid for simultaneous estimation of doxycycline in turkey serum samples.

DOI

10.21608/ejceh.2016.248064

Keywords

validation, Doxycycline, serum, HPLC 

Authors

First Name

Nahla

Last Name

Elshater

MiddleName

S.

Affiliation

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute,Dokki

Email

-

City

-

Orcid

-

First Name

Fatma

Last Name

Hussein

MiddleName

-

Affiliation

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute,Dokki

Email

-

City

-

Orcid

-

First Name

Mai

Last Name

Fadel

MiddleName

A

Affiliation

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute,Dokki

Email

-

City

-

Orcid

-

First Name

Marwa

Last Name

Ragab

MiddleName

-

Affiliation

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute,Dokki

Email

-

City

-

Orcid

-

First Name

Heba

Last Name

Hassan

MiddleName

-

Affiliation

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute,Dokki

Email

-

City

-

Orcid

-

First Name

Maha

Last Name

Sabry

MiddleName

-

Affiliation

Chemistry Department, Animal Health Research Institute,Dokki

Email

-

City

-

Orcid

-

Volume

2

Article Issue

2

Related Issue

33292

Issue Date

2016-11-01

Receive Date

2022-07-05

Publish Date

2016-11-01

Page Start

282

Page End

291

Online ISSN

2357-1039

Article File

EJCEH_Volume 2_Issue 2_Pages 282-291.pdf

PDF . 432.9kB

Link

https://ejceh.journals.ekb.eg/article_248064.html

Detail API

https://ejceh.journals.ekb.eg/service?article_code=248064

Order

248,064

Type

Scientific and Research

Type Code

2,340

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry and Environmental Health

Publication Link

https://ejceh.journals.ekb.eg/

MainTitle

Validation of HPLC Method for Determination of Doxycycline hyclate in turkey serum

Details

Type

Article

Created At

23 Jan 2023