Microbial fuel cells (MFCs) are considered simple and robust technology in the field of wastewater treatment as well as electricity generation. The middle-term application can be foreseen at market value prices. This technology might become certified as a prospective new technology for the conversion of organic wastes to electricity. In the current study, a total of 40 yeast isolates were collected from 20 samples of sugarcane molasses. Twenty-one yeast isolates were examined for their potential to produce electricity from sugarcane molasses. All isolates had the ability to produce electricity. Four of them (isolates number: MY 9, MY10, MY18 and MY19 were considered as high producers. They yielded power densities of 59.858, 61.25, 52.8125 and 47.432 mW/m², respectively. Yeast isolate MY10 from sugarcane molasses showed the highest power density (P) (61.25 mW/m²), highest current (I) (0.175mA/m²) and highest voltage (V) (350mV/m²). This isolate was identified based on 18S rRNA sequences as Meyerozyma guilliermondii and deposited in the Gene bank nucleotide sequence database under accession numbers KY624417.