The present study was designed to investigate the effect of feeding different levels (10 & 20 & 30%) of fresh broccoli on liver and kidney functions, serum lipid profiles, blood sugar, serum total protein and albumin. In addition to determined of bioactive compounds (flavonoid and saponine). Thirty adult Sprague-Dawley strain male healthy albino rats weighting (80±5g) were used as experimental animals and divided into 5 groups (6 rats each). The experimental animals (30 rats) were fed on basal diet for one week to adapt with the basal diet. After that, rats (30) were divided into two main groups. The first group (6 rats) was fed on basal diet for 7 weeks to be as a negative control group. The second group (24 rats) was divided into 4 sub-groups (6 rats each). The first sub-group (B1) was fed on hypercholesterolemic diet (diet containing 10% animal fat+1% cholesterol) for 7 weeks and was left as a positive control group (untreated group). The other sub-groups were fed on hypercholestrolemic diet for 3 weeks to be as hypercholesterolemic rats. After that, animals were fed on diet supplement with different levels (10&20 &30 %) of fresh broccoli for 4 weeks. At the end of the experimental period animals were killed, blood samples were collected to determine the following parameters, blood sugar, lipid profiles, liver functions, serum total protein, albumin, kidney functions (urea, uric acid and createnine). Liver, kidney were taken for histopathological examination. The obtained results concluded that, 30% levels of fresh broccoli improve of all biochemical parameters.