Two field experiments were carried out at The Experimental Farm, faculty of Agricultural at Dar El Ramed, Fayoum University, Egypt, during two winter seasons of 2015/2016 and 2016/2017. The objectives of experiments were to assess the effect of replacing part of chemical N fertilizers through the organic, bio, and foliar application of organic stimulants on growth parameters (fresh weight of shoots per plant (g), fresh weight of roots per plant (g), dry weight of shoots per plant (g), dry weight of roots per plant (g), Plant height (cm) and chemical composition (plant pigments), macro elements content and enzymatic activities (dehydrogenase activity (μg TPF/ g dry soil/ day) & Nitrogenase activity (μ mole C2H4 / g rhizosphere /h)}, of wheat plants (Triticum aestivum L.) cv. Sids12. The experiment comprising eleven treatments in three replications was laid out in a randomized block design. Results indicated that, wheat growth criteria (fresh weight of shoots per plant (g), fresh weight of roots per plant (g), dry weight of shoots per plant (g), dry weight of roots per plant (g), Plant height (cm), chlorophyll content of leaves, N, P, and K   uptakes in the flag leaf of wheat plants with all treatments receiving bio- fertilizer +  50% N- compost + 50% N-mineral treatment showed the highest significant increase in growth criteria followed by  50% N-mineral +50%N- compost + foliar application of mix organic acids. Combined inoculation of mixture bio-fertilizers with 50% N-mineral +50%N- compost (T9) gave the highest nitrogenase and dehydrogenase activities in the rhizosphere as well as the macronutrient uptakes in the flag leaf of wheat plants. The highest significant increases for all growth characters and yield components of wheat plants were also recorded by the same treatment (T9). It could be concluded that there is a possibility of using microbial inoculations (Azotobacter and Azospirillium) with the addition of organic manure to soil in order to reduce the amount of mineral fertilizers and to produce a good yield and quality of wheat in addition to improve the biological properties of the soils.