Four types of probiotics were prepared from local isolates to study some factors effected which one was more resistance to acidity and bile salts. The ability of probiotics were tested for acidity resistance. Results indicate that the probiotic (3) was gave the highest survival rate 99.1% at pH 5 after 3 hour of incubation 37C°. Results also showed that the probiotic (3) was more resistance to bile salts at all concentration and resulted in 88.4, 66.3 and 5.6% survival rate at 0.1, 0.2 and 0.3 bile salts respectively, finally, probiotic (3) was selected as the best and safe probiotic, so it can use to support immune system .The goal of project is to preparate and test local probiotics resistant for some concentration of bile salt and acidity.