Toxocara vitulorum is a pathogenic gastrointestinal nematode found in cattle and buffaloes all over the world, especially in tropical and subtropical regions with humid climates. Buffalo farming is a sector whose value has increased in recent years in Turkey and is intensively performed to obtain various products such as milk, cream, meat, and horns. This study aims to determine the prevalence of T. vitulorum in Anatolian Water Buffaloes in Diyarbakır. Fresh stool samples from animals were placed in individual stool containers. The sex and age of the animal were recorded for each sample collected. The samples were evaluated under the light microscope after the application of the Fulleborn saturated salt solution method and under Scanning Electron Microscope. Positivity was detected in 5 (3.01%) of the 166 samples. Regarding prevalence by age groups, T. vitulorum was detected in 10% of the 0-6 month group, 4.17% of the 6-12 month group, and 0.89% of the older than 12 months group. The prevalence by sex was determined as 3.85% for females and as 1.61% for males. To determine the epidemiology of the disease in the region, it was concluded that larger herds should be studied and serological and molecular methods should be used to confirm and support the findings of microscopic methods.