A total of 51 samples of fresh water fishes 26 Tilapia nilotica. 5 Clarias lazera. 5 Mormyrus caschive. and Synodontis membranaceus were collected from Assiut City markets and examined. The average number of viable count per gram muscle of Tilapia nilotica The Clarias lazera (CI), Mormyrus caschive Mc, ana Synodontis membre naceus (Sm) were 78.7x10785.6x107, 76.8x107 and 50.4x 107 respec tively. 42 isolates could be isolated from the total specimens exami ned and identified as follows: 1.98% Enteropathogenic Escr.com (E.P.E.C.) 044/K74, 1.98% Serratia rubidaea, 1.98% Edwarasiella. 3.92% Enterobacter cloacae, 5.88% Strept. faecalis, 7.84% Aeromonas panctata, 11.76% Enterobacter aerogenes, 11.76% proteus morganii, 11.76% Ps. diminuta and 23.53% Ps. aeruginosa,