One hundred and twenty random samples of some selected food items, including, raw milk, Damiętta and Kareish cheese, hamburger and fresh sausage, collected from different localities in Assiut City, were sereened for fEnterobacteriaceae. The obtained results revealed that 70, 35, 80, 90 and 35% of the examined samples, respectively, were contaminated by Enterobacteriaceae. The different isolates recovered from the examined food samples were identified as E.coli; Enterobacter hafniae and liquefacient; Citrobacter diversus and freundii; Alkaligenes faecalis; Klebsiella ssp. Providencia spp.; End wardsiella tarda; Proteus mirabilis, rettergi and Vulgaris; Acinetobactr spp.; Serratia liquefaciens and marcessens; Salmonella spp.Arizona hinshawii and Pseudomonas aerogenosa. The public healths and suggestive measures were discussed.