A known viable population of the washed inoculum of either Cl.chauvoei or Cl.septicum (6x10 spores or vegetative cells/gm soil) was added to three types of soil (dairy farm soil, cultivat ed soil as well as sandy soil).
soil
and
At predetermined times, bottles representing each control were subjected to bacteriological examination.
About 80-90% of the inoculated vegetative cells of Cl.chauvoei and Cl.septicum sporulated within 72-96 hours. The rate of Cl.chauvoei sporulation was slightly rapid than Cl.septicum. Moreover the spores of the two organisms persisted in the different soil from 10-16 months.