different samples of kareish cheese were collected from random Fifty markets in Alex. City, were examined for incidence and enumeration of coliform organisms. Ninty-two percent. of the examined kareish cheese samples proved to be contaminated with coliform organisms with a mean value (MPN / 100g) of 6.9X10'. Escherichia coli could be isolated from 34.0 % of examined samples, while Enterobacter Spp; Citrobacter Spp. and Klebsiella Spp. could be also isolated at varying percentages. The serological typing of isolated E. Coli strains were: 0124: K72: B17; 026: K60: B; Oss: Ks: Bs; 0119: K64:B14; 08: K61: B; ; 0126: K71: B16 and 012: K70: Bis. The electrophoretic pattern of the proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS - PAGE) prepared from the cell wall of the different isolated strains of E. Coli revealed that several bands differ in migration position and molecular weights which differ among the different strains. Each one of them has a specific characteristic feature in the protein bands and concentration.