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201181

STUDIES ON A RECENT IBD VIRUS FIELD VARIANT ISOLATE: (3) IMMUNOGENICITY OF THIS VARIANT STRAIN COMPARED WITH A CLASSICAL STRAIN IN BROILER CHICKENS

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Last updated: 05 Jan 2025

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Abstract

SUMMARY   Three inactivated water–in–oil–in–water–emulsion infectious bursal disease virus (IBDV) vaccines were prepared from the Del/E related IBDV variant strain and a classical vvIBDV strain. Each IBDV strain were propagated for 6 serial passages in specific pathogen free (SPF) embryonated chicken eggs (ECE). The harvested IBD viruses were titrated in 10 day old ECE and inactivated using formalin at a final concentration of 0.2 % of the total volume. The fist vaccine contained  Del/E related IBDV variant strain, the second vaccine contained vvIBDV strain and the third vaccine contained both variant and  vvIBDV strain. The 3 prepared IBDV vaccines are evaluated compared to a commercial inactivated IBDV vaccine, all the 4 vaccines proved to be sterile and safe. The efficacy of each vaccine was assisted by inoculation into a group of 14 day old broiler chicks via subcutaneous rout and challenged (2 weeks later) with Del/E related IBDV variant strain and the classical vvIBDV strain. The humeral immune response were determined using indirect ELISA, the geometric mean (GM) ELISA titer of the vaccinated chicken groups are significantly higher than the non vaccinated chickens, with no significant difference between the vaccinated chicken groups despite the variation in the IBDV strains. The protection was evaluated at 5 and 10 days post challenge, based on gross and microscopic lesions, bursal indices, proventricular/body weight ratio and bursl lesions scores. Vaccines made of variant IBDV alone or combined with vvIBDV protected chickens challenged with either the classical vvIBDV or the Del/E related IBDV variant strain while vaccines made of the classical IBDV strain alone protected chickens challenged with the classical vvIBDV strain but not against the Del/E related IBDV variant strain.

DOI

10.21608/avmj.2005.201181

Keywords

Key words: Vaccination, IBD virus, Broiler chickens

Authors

First Name

S.A.

Last Name

NASSIF

MiddleName

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Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo

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City

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Orcid

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First Name

ELHAM

Last Name

A. EL-EBIARY

MiddleName

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Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo.

Email

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City

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Orcid

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First Name

K.S.

Last Name

SHAABAN

MiddleName

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Affiliation

Faculty of Veterinary Medicine, Cairo University

Email

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City

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Orcid

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First Name

S.S.

Last Name

ABDEL–RAHMAN

MiddleName

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Affiliation

Veterinary Serum and Vaccine Research Institute

Email

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City

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Orcid

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First Name

M.M.

Last Name

AMER

MiddleName

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Affiliation

Faculty of Veterinary Medicine, Cairo University

Email

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City

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Orcid

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Volume

51

Article Issue

107

Related Issue

24406

Issue Date

2005-10-01

Receive Date

2005-09-29

Publish Date

2005-10-30

Page Start

1

Page End

15

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_201181.html

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https://avmj.journals.ekb.eg/service?article_code=201181

Order

23

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

STUDIES ON A RECENT IBD VIRUS FIELD VARIANT ISOLATE: (3) IMMUNOGENICITY OF THIS VARIANT STRAIN COMPARED WITH A CLASSICAL STRAIN IN BROILER CHICKENS

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Type

Article

Created At

23 Jan 2023