During late autumn and winter, the period of December 2004- February 2005, 4 ostrich farms around Egypt were surveyed and samples from 54 clinically diseased and fresh dead birds were collected and tested for detection and determination of the incidence, course, clinical signs, PM, histopathology and the pathogenicity of NDV among ostriches (Struthio camelus). Two NDV isolates were detected, propagated in chicken embryos, pathotyped and examined by EM. The present retrospective study was prompted by the finding of HI was adopted for the detection and quantification of antibodies to NDV. Abbreviations: CIAF = clarified infected allantoic fluid; CRBCs= chicken red blood cells; ECEs = embryo chicken eggs; EID50 = median embryo infective dose; ELISA = enzyme linked immunosorbent assay HA = haemagglutination; HI = haemagglutination inhibition; IAF= infectious allantoic fluid; ICPI =.intracerebral pathogenicity index; IVPI = intravenous pathogenicity index; LPB-ELISA = liquid phase blocking enzyme-linked immunosorbent assay, MDT = Mean death time; ND = Newcastle disease; and NDV = Newcastle disease virus.