In this work seventeen balady sheep were divided into 6 groups, the first group (G1) was vaccinated S/C with binary inactivated RVF vaccine with 50% IMS 3013, the second group (G2) was vaccinated S/C with binary inactivated RVF vaccine containing 0.5% saponin with 50% IMS 3013, the third group (G3) was vaccinated S/C with binary inactivated RVF vaccine with 25% IMS 3013, the fourth group (G4) was vaccinated S/C with binary inactivated RVF vaccine containing 0.5% saponin with 25% IMS 3013, the fifth group (G5) was vaccinated S/C with inactivated RVF vaccine with aluminum hydroxide gel while the sixth group (G6) left as a control. The results revealed that RVF inactivated vaccine with IMS3013 either 50% or 25% give higher level of antibody and reaching its protective level earlier than RVF inactivated vaccine with aluminium gel and the best vaccine is RVF inactivated vaccine containing 0.5% saponin with 50% IMS 3013 where ED₅₀ equal 0.0008/ml and gave a higher level of antibody all over the period of the test compared with that of other vaccinated groups when tested by lymphocyte transformation assay using MMT staining procedure and serum neutralization test.