Five non pregnant, non lactating she-donkeys of about 5 years in age and 250 kg in weight were used in this investigation. Animals were tied under shed in an open yard with a concrete floor belonging to the experimental farm of faculty of veterinary medicine, Assiut University. They were ad libitum fed a maintenance ration. Drinking water was freely available allover the experiment. Donkeys were subjected to four experimental trials, two weeks each (bedding the floor, offering Barseem hay with the ration, watering each two days and housing inside closed pens). Behavior of the experimented animals was recorded. Moreover, their health status and some of blood parameters were estimated. The obtained results indicated that, managemental factors related to the time spent in the stable showed the strongest associations with stereotypic and abnormal behavior. Some of these conditions were considered as a stressful factor and reflected prominently on the animal's health status and blood parameters. Therefore, changes in the management program within the equine farms should be avoided to provide the animals with a comfortable situation.