Total proteins, albumin, globulin, A/G ratio, alkaline phosohatase (AP), aspartate aminotransferase (AST), alanine aminotronsferase (ALT) and cholesterol were studied in fourteen buffaloes, seven of them during estrus cycle and the other buffaloes remained anestrus for 90 days or more postpartum. Total proteins, albumin, globulin, and cholesterol levels were high at estrus comparsion with other days of estrus cycle and anestrus period, these differences were significantly (P<0.05). A/G ratio was fluctuated throughout of estrus cycle. Value of A/G ratio in anestrus period was low and similar to 8 and 10 days of estrus cycle comparison with other days of estrus cycle. Alkaline phosohalase activity was low at estrus in comparison with anestrus period and other days of estrus cycle, these differences were significantly (P<0.05). Concentration of AST was high at estrus in comparison with other days of estrus cycle and anestrus period, it was statistically non-significant. ALT activity was high at the end of estrus cycle in comparison with other days of estrus cycle and anestrus period, these differences were significant (P<0.05).