The present study aimed to evaluate the efficacy of ultrasound in diagnosis of obstructive urolithiasis and rupture of the urinary bladder in cattle and buffalo. The study was carried out on 30 non castrated cattle and buffaloes males (19 cattle and 11 buffaloes), suffering from obstructive urolithiasis. Case history and clinical signs were recorded. Diagnosis was confirmed by radiography and surgery. Ultrasonographic examination showed urethral pulsation and urethral dilatation in front of the seat of obstruction as well as the changes in the wall thickness of the urinary bladder and urethra due to rupture, inflammation or obstruction, Ultrasound allowed diagnosis of cystitis in 19 cases, where the bladder wall was thickened, and the urine was turbid. Ruptured urinary bladder was visible in 12 cases. The site of the rupture in the urinary bladder was visible in 4 cases. Free abdominal fluid due to rupture of the urinary bladder was visible in 20 cases. In 8 of these cases, the urinary bladder healed spontaneously after rupture and was intact and distended with urine during the ultrasound examination.