Seventeen Balady sheep were used in this investigation. The animals were divided into 6 groups, the first group (G1) was vaccinated subcutaneously (S/C) with binary inactivated Rift Vally Fever (RVF) vaccine with 1.00% aluminium phosphate, the second group (G2) was vaccinated S/C with binary inactivated RVF vaccine with 0.75% aluminium phosphate, the third group (G3) was vaccinated S/C with binary inactivated RVF vaccine with 0.50% aluminium phosphate, the fourth group (G4) was vaccinated S/C with binary inactivated RVF vaccine with 0.25% aluminium phosphate, the fifth group (G5) was vaccinated S/C with inactivated RVF vaccine with 2% aluminium
hydroxide gel while the sixth group (G6) left as control. The results revealed that group (1) followed by group (2) gave higher level of antibody and reaching its protective level earlier than RVF inactivated vaccine with aluminium gel and the duration of immunity of aluminium phosphate when compared with that of aluminium hydroxide is mush longer. In addition, the best vaccine was RVF inactivated vaccine containing 1.00% aluminium phosphate followed by RVF inactivated vaccine containing 0.75% aluminium phosphate as they gave higher level of antibody all over the period of the test compared with that of other vaccinated groups when tested by serum neutralization test and ELISA test. Moreover, manufacturing of these vaccines is easy to be done and of low cost.