The present study aimed to investigate the bacterial causes of mortality in newly born rabbits and symptoms and pathological lesions. A total of 200 kits (120 freshly dead kits, 40 diseased kits and 40 apparently healthy kits) were collected from private farms at Dakahlia Governorate. The symptoms of diseased rabbits were depresion, weakness, diarrhoea and respiratory distress, while P.M. were enlarged liver and spleen, hemorrhage on the internal organs and congested lungs. The bacteriological examination revealed 60 cases (47.62%) and 66(52.38%) were single and mixed infection respectively. E.coli was isolated at incidence percentage 34 (17.71%), each of Pasteurella multocida and Salmonella 28(14.58%), Klebsiella pneumoniae 27(14.06%), Staphylococcus aureus 32(16.67%), Streptococcus pyogenes 25(13.02%) and Pseudomonas aeruginosa 18 (9.38 %). E.coli isolates were identified serologically into 12(O₁₂₆K₇₁(B16) and 7each from (O₂₆K₆₀(B6), (O₅₉K₅₉(B1). Also Salmonella spp. was identified as 14 Salmonella typhimurium and 8 Salmonella enteritidis. In vitro sensitivity pattern of isolated strains proved that Enrofloxacin and Gentamycin were the most effective drugs for most isolates.