In this study 100 cases of pigeons were collected from differents localities at Sohag Governorate. 6 positive cases of Ps.aeruginosa were isolated with an incidence of 6%. The experimental infection in one- month-old squabs by different routes showed that subcutaneous route was highly effective with mortality rate 100%, while oral route resulted in 60% mortality. In vitro antibiotic sensitivity test revealed that the examined isolates were highly sensitive to amikin, enrofloxacin, polymyxin B, colistin sulfate and gentamycin and were resistant to ampicillin, erythromycin and oxytetracyclin.