175977

APPLICATION OF IMMUNOHISTOCHEMISTRY TO DETECT CAMELPOX VIRUS IN CELL CULTURE AND HISTOLOGICAL SECTIONS

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Last updated: 05 Jan 2025

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Abstract

During the spring and summer of 2005, an outbreak of camelpox was spread in the province of Hama and Duma city. Therefore, this study was designed to detect camelpox virus in Vero cell culture and paraffin tissue section by immunohistochemistry. Skin samples (pustules, vesicles and scabs) from infected camels were collected from Duma and Hama. Monoclonal antibody (mouse anti-camelpox IgG) and labeled secondary antibody (rabbit anti-mouse IgG HRP) were used to demonstrate camelpox virus. Camelpox virus was localized in the cytopathic effects in cell culture (syncytia and round cells) and inclusion bodies in the dermal and epidermal cell of the skin. The present study shows that immunohistochemistry is a sensitive diagnostic technique to detect the infection in skin samples with sensitivity of 98% and reflects its effectiveness and accuracy in camelpox infection diagnosis.  

DOI

10.21608/avmj.2007.175977

Keywords

Key words: Immunohistochemistry, camelpox virus, histology

Authors

First Name

OMAR

Last Name

ALZIABI

MiddleName

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Affiliation

Faculty of Veterinary Medicine, Hama, Syria

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Orcid

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Volume

53

Article Issue

112

Related Issue

24393

Issue Date

2007-01-01

Receive Date

2006-12-13

Publish Date

2007-01-01

Page Start

209

Page End

222

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_175977.html

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https://avmj.journals.ekb.eg/service?article_code=175977

Order

16

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

APPLICATION OF IMMUNOHISTOCHEMISTRY TO DETECT CAMELPOX VIRUS IN CELL CULTURE AND HISTOLOGICAL SECTIONS

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Article

Created At

23 Jan 2023