In the present study, 505 of chicken (185 native breed chickens and 320 fating breed) in addition to 50 soil samples (forty soil samples from around farmer houses and 10 from around poultry farms) were examined for epidemiologicalstudy of Ascaridia galli in Assiut Governorate. The infection rate of A. galli in chickens was (3.17 %) where in native breed chickens was and it was not detected in fatting breed. The highest infection rate was detected in winter season (4.03 %). The rate of positive soil samples from around farmer houses was (12.5 %) for A.galli eggs and no eggs were encountered in soil samples collected from around poultry farms. Developmental stages of A. galli eggs were studied. Embryonation of eggs started at the third day and complete embryonation with the development of larvae within egg shell occurred on the 16^th day. Second stage larvae of A. galli were described and microphotographed, they were obtained by using of magnetic stirrer. Experimental infection of laboratory mice with fully embryonated eggs of Ascaridia galli was done per mouth. Pathological features indicating larval invasion of the intestine, liver and lung were noticed. This indicated that A. galli might be transmitted to humans specially children by eating raw vegetables contaminated with soil and causing visceral larval migrans.