A total of 100 buffalo meat samples were collected from different markets in
Giza govemorate. They were subjected to physical and bacteriological
examination. Thirty nine percentage of the samples tested were found to be
contaminated with Clostridium perfringens type A. The most probable
number (MPN/gram) values ranging from 0 to 35. Also, the spore count of
Clostridium perfringens type A at 100°C at different time interval was
investigated. Electrophoretic analysis and immunobloting for detection of
Clostridium perfringens enterotoxin type (A) was carried out for its
characterization. The immunogenic band was determined with molecular