Examination of different samples from 750 chicken cases of various growth stages, revealed the isolation of 42 Salmonella isolates (5.75%). The isolated salmonellae were serotyped as Salmonella enteritidis       (20 isolates), Salmonella typhimurium (13 isolates), Salmonella infantis (5 isolates), Salmonella montivideo (3 isolates) and Salmonella cerro    (1 isolate). Antibiogram of the isolated Salmonellae indicated multidrug- resistance to one or more than of the tested antimicrobial agents. Pathogenicity tests in one-day old chicks proved the virulence of all examined serovars with various degrees of pathogenicity and all were positive for congo red activity. SDS-PAGE protein analysis of different serovars revealed 6-10 protein bands ranged from 13.631-200.5 KD, which in relation to the isolated Salmonella serovar.  Immunoblotting of the isolated serovars revealed the presence of common protein bands at 15, 29 and 35 KD. The highest antigenicity protein band of S. typhimurium was detected at 35.4 KD, while in S. enteritidis was detected at 29KD; in S. montivideo at 39.8 KD; in S. infantis at 25.128 and 34.091 KD, while S. cerro had the highest antigenic protein band at 143.08KD. In conclusion, SDS-PAGE analysis and immunoblot provide a recent and accurate techniques for detection of salmonellosis in chickens, in addition to offer the use of immunogenicity of different detected immunogenic bands to serve as components of an effective subcellular vaccine for poultry salmonellosis.