Selenium was investigated in serum and tissues (liver, kidney and muscles) of 246 animals (73 cows, 74 buffaloes, 37 camels and 62 sheep & goats) ranging from less than 1 year to 7 years (1.5-6 for cows, <1-7 for buffaloes, 3-7 for camels and 1-4 for sheep & goats), slaughtered in Ismailia slaughterhouse. Results revealed that selenium levels in the blood serum doesn't significantly differ (P>0.05) between sexes except for sheep and goats, where females had significantly higher levels than males (P<0.05). Serum level of selenium was considered low in 95.9% of buffaloes, 82.9% of cows, 100 % of camels and 90.9% of sheep and Goats. Level of selenium in tissues of females were significantly (P<0.05) higher than male in buffalo, sheep and goats, but not in cow (P>0.05). Concerning the level of selenium in the liver, all cows, sheep and goats and 66.7% of buffaloes were concerned as low level. No distinct symptoms of nutritional myopathy were observed in the slaughtered animals. Most of the animals slaughtered in Ismailia slaughterhouse showed low levels of selenium and need to be supplemented with selenium in their ration.