A total of 220 raw milk samples were collected randomly from Beni-Suef and Ehnasia cities in Beni-Suef Governorate, comprising 170 small dairy herd and 50 retail milk samples. All the samples were examined serologically for brucellosis using MRT, wRBPT, w BAPAT, wTAT and w Riv.T. The results revealed that 14 (8.2%), 20 (11.8%),              24 (14.1%), 4 (2.4%) and 7 (4.1%) out of 170 small dairy herds milk samples were positive respectively. On the other hand, 3 (6%), 5 (10%), 8 (16%), 1 (2%) and 0 (0%) out of 50 retail milk samples were positive for MRT, wRBPT, w BAPAT, wTAT and w Riv.T respectively. The results indicated that the milk of small dairy herds and retailers in both examined cities play a dangerous role in transmitting infection to human and constitute a public health hazard. The necessary measures to control the disease were discussed