Seventy five random samples of milk and some dairy products including raw milk, small scale ice cream and Talaga cheese (each of 25) were collected from different localities in Bani-Suef city. Pseudomonas species could be counted, isolated and identified on cetrimide agar and GSP agar at different counts and percentages, also the incidence and count of psychrotrophic organisms was determined. The characterization of some isolated pseudomonas spp. from the examined samples for the production of extracellular virulence factors as lipolytic and proteolytic enzymes, were detemined and from that 71 pseudomonas spp. showed lipolytic activity while 70 isolates of them showed proteolytic activity. The public health significance of the organism and the precautions, which should be taken to control this organism in the dairy industry as well as the recommended sanitary measures, were also discussed.