ِA serological survey was conducted in attempt to detect the antibodies against border disease (BDV) in sheep and goats at AL-Ahsa region, Kingdom of Saudi Arabia. During the period 2006-2007, a total of 1471 serum samples were subsequently collected and serologically tested by using a commercial ELISA kit, which revealed that 23.72% and 13.11%  of examined sheep (n=548) and goats (n=923) were seropositive to BDV infection, respectively. The offered data declare a strong correlation between the age of the tested animals and seroprevalence of BDV infection. The data also revealed highly significant increase (P< 0.07) in the susceptibility rate of sheep to BVD infection than goats. There is no significant effect of sex. The effect of breed on goats is also non-significant, but breeds of sheep varies significantly in the serosurvey of border disease virus antibodies as the Awassi is the higher and the barbari is the lowerest. It can be concluded that BDV is serologically established in sheep and goats in Al-Ahsa region in Kingdom, Saudi Arabia.