In this study experimental infection of mature rabbit bucks was carried out by the isolated bacteria from field cases. The gross and histopathological changes in testes in addition to semen characteristics were evaluated. Total twenty one rabbit bucks used in this experimental study were divided into 3 groups (6 in each), the groups, first, second &third were inoculated subcutaneously(s/c) in scrotum with Pseudomonas aeruginosa, Pasteurella multocida and both Pseudomonas aeruginosa and Pasteurella multocida in order. The remaining 3 bucks were left as non infected control. Infection with Pasteurella and Pseudomonas had more deleterious effects than either Pasteurella or Pseudomonas alone on semen quality and testicular tissues. All affected groups had differed significantly from control; volume of semen /ml was decreased significantly (p‹ 0.01), concentration x 10⁶/ml of spermatozoa and percentage of motile sperm were decreased significantly (p‹ 0.001). Percentage of dead spermatozoa, major defects %, minor defects % and acrosomal defects (semen) were increased significantly (p‹0.001). Also the acrosomal defect from epididymis was increased significantly (p‹0.01). The third group was severly affected than first and second groups. The infected rabbit bucks with Pseudomonas aeruginosa and Pasteurella multocida either single or both resulted in moderate to severe gross scrotal inflammation, which lead to damage of the testicular parenchyma with different degrees of severity. Histopathological examination revealed less intense lesions in bucks infected with Pseudomonas aeruginosa compared with those infected with Pasteurella multocida or both organisms, in which the semeniferous tubules showed excessive necrosis and progressive to complete depletion of spermatogoneal cells.