Bacteriological examination of 370samples which were taken from organs offreshly dead and diseased chickens,ducks and turkeys showing diarrhoea and respiratory disorders (collected from different farms at Beni Suef Governorate) revealed isolation of 34(21.25%), 38(29.23%) and 14(17.50%) isolates of P. multocida respectively and 19 isolates of Mannheimia hemolytica of which 16(10%) isolates from chickens and 3(3.75%) isolates from turkeys. The biological characters of both P. multocida and Mannheimia hemolytica isolates were discussed. PCR based technique was used in identification of P. multocida, all examined isolates gave a single amplified product of the expected size 460 bp.Antibiogram study showed that P. multocida and Mannheimia hemolytica isolated from chickens,ducks and turkeys were sensitive to ceftiofur, gentamycin, lincomycin + spectinomycin,and norfloxacin .The present study indicated that the recent development of species-specific PCR assay for P. multocida has provided rapid sensitive and specific method for identification of P. multocida isolated from chickens,ducks and turkeys.