Parasitological examination of faeces, gall bladders and livers as well  bacteriological examination of gall bladders and livers of cows and buffaloes, revealed the presence of some parasitic and bacterial infections either single or mixed infections (Fasciola with bacteria or with other parasites in one animal). Faecal examination of 218 cows, indicated that 8.26%, 11.93%, 11% and 1.83% cases were infested with Fasciola, Gastro Intestinal Parasites (G.I.P.), Paramphistomum and Coccidia respectively. Also from 167 buffaloes faecal samples 5.99%, 10.18%, 9.58% and 1.20% were infested with Fasciola, Gastro Intestinal Parasites, Paramphistomum and Coccidia respectively. There were higher rates of infestations with all mentioned parasites in cows than buffaloes. As for mixed infections, 12 from 18 fasciolised cows had faecal-borne parasites (66.67%), 5 of which were infested with Gastro Intestinal Parasites (27.78%) and the other 7 cows had paramphistomiasis (38.89%). Regarding buffaloes 3 from 10 (30%) had Fasciola and Gastro Intestinal Parasites beside 2 from 10 (20%) had Fasciola and Paramphistomum. Bacteriological examination of 110 samples of gall bladders and inflamed livers of cows and buffaloes terminated 88 positive samples, 49 of them were mixed with liver fluke infestations and 39 were pure bacterial infection. Different species of bacteria were detected in the examined samples at different rates as follow: Fusobacterium necrophorum (13.40%), E. coli (12.37%,), Serratia marcescens (11.34%), Pseudomonous aeruginosa (10.31%), Enterobacter aerogenes (9.28%) Actinomyces pyogenes (8.25%) Staphylococcus aureus (7.21%), Streptococcus uberis (6.19%), Enterococcus faecalis (6.19%), Proteus vulgaris (6.19%), Enterococcus faecium (5.15%) and Proteus mirabilis (4.12%). Serological identification of the isolated E. coli determined that 4 strains were O111, 4were O29, 3 were O26 and one strain was O164. Antimicrobial susceptibility of the isolated strains of E. coli, S. aureus and Strept. uberis recovered from the infected livers to different chemotherapeutic agents was discussed and the minimum inhibitory concentrations (MIC) for the isolates of F. necrophorum and A. pyogenes to some antimicrobial food additives were determined.