The aim of the present study is to investigate the effect of post parturient haemoglobinurea (PPHU) on haematological and biochemical parameters of cattle excessively fed on Trifolium Alexandrium (Barseem) during the green season in newly reclaimed area. Blood and urine samples were collected from 40 diseased cattle aged from 6-9 years showing signs of loss of appetite, refuse barseem eating, red to coffee coloured urine and milk production dropped rapidly. A number of 15 clinically healthy cattle were used as control group. The haematological studies showed significant decrease in TRBCs, Hb concentration, PCV and significant increase in TWBCs. The blood hydrogen ion concentration (pH) and bicarbonate were found non significant increased. Blood gas analysis showed significant increase in PCO2 and significant decrease in PO2. Serum biochemical analysis revealed that cattle with PPHU was accompanied by a marked decrease in total protein, albumin, globulins, glucose, total cholesterol, total triglycerides, inorganic phosphorus, magnesium, sodium, potassium, zinc, iron and copper levels. Moreover significant increase in AST, ALT, ALP activity levels, blood urea and molybdenum levels was noticed when compared with apparent clinically healthy ones. Moreover a marked increase in the excretion of phosphorus, calcium, molybdenum, protein, urea and creatinine through urine was observed only in PPHU affected cattle. Concerning the diet, analysis of barseem grows in newly reclaimed area, the obtained values of copper and calcium was adequate while phosphorus showed significant decrease levels. Molybdenum level has an opposite direction to copper level and showed highly significant increases in its values when compared with barseem grow in old village. The affected PPHU cattle were treated according to the severity of the case by sodium acid phosphate and tonophosphan injection. Copper glycinate was also tried to halt haemolysis. Complicated cases were completed cured after blood transfusion. The symptoms disappeared, the appetite returned to normal and haematological as well as biochemical measured parameters in serum and urine returned nearly to normal. So attention must be paid for careful early clinical and laboratory diagnosis of diseased animals followed by therapeutic plan with good management. Further more excessive feeding on barseem must be avoided and feeding balanced ration mixed with minerals, trace elements and vitamins during the green season.