IL-8 is a neutrophil chemoattractant that plays important role in the regulation of inflammatory responses. The role of IL-8 in the Staphylococcus aureus mastitis has drawn considerable attention in the last years. The level and extent of the IL-8 transcriptional activity was considered vital to be monitored. Bovine TaqMan^® real-time PCR was employed to examine the IL-8 mRNA transcriptional activity in cattle experimentally infected with Staphylococcus aureus at the time points  0, 7, 24 and 32 hrs post-infection. The level of IL-8 transcriptional activity increased at 24 hrs post-infection, however non-significantly. The non-significant increase in the IL-8 activity in Staphylococcus aureus mastitis was seen in accordance with results that several studies have reported recently. It was postulated that Staphylococcus aureus elicits immunosupression by dwonregulating the NF-κB, a major IL-8-inducing factor