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174623

EFFECT OF NISIN AND CINNAMON ON THE GROWTH AND SURVIVAL OF ENTEROTOXIGENIC METHICILLIN-RESISTANT STAPH. AUREUS IN STERILE MILK AND ICE CREAM

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Last updated: 05 Jan 2025

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Abstract

  The effect of nisin on the survival of enterotoxingenic methicillin-resistant Staph. aureus (MRSA) was evaluated using different concentrations of nisin (0.00, 100 and 200 IU/ml) in a laboratory prepared sterile milk and inoculated with the previously isolated and identified MRSA to yield a concentration of 1x107 cfu/ml. The inoculated sterile milks were kept at room temperature (20±2ºC) and refrigerator temperature (4±2ºC). MRSAcounts were determined using Oxacillin Resistance Screen Agar Base (ORSAB) supplement with two antibiotics-oxacillin at (2 mg/L) and polymyxin B (50.000 IU/L). ORSAB and pH value were determined every twelve hours. MRSA strains couldn't be detected after 96 and 72 h in the samples of sterile milk containing nisin in concentrations of 100 and 200 IU/ml, while, in the control sample the MRSA survived till the end of the 96 h of storage at room temperature. Moreover, MRSA failed to be detected after 48 and 24 h in the samples of sterile milk containing nisin in concentrations of 100 and 200 IU/ml and storaed at refrigerator temperature, respectively. Ice cream was prepared at the laboratory to study the effect of ground cinnamon in concentrations of 0.3 and 0.6% on the growth and survival of enterotoxingenic MRSA a concentration of 2x107 cfu/ml at freezing  (-4±2ºC) and deep freezing (-18±2ºC) temperatures. The obtained results showed that the advantage of using 0.6% is better than using of 0.3% of ground cinnamon.

DOI

10.21608/avmj.2009.174623

Keywords

Key words: Enterotoxigenic MRSA, Milk, Nisin, Ice cream, Cinnamon

Authors

First Name

S.

Last Name

NASR

MiddleName

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Affiliation

Dept. of Food Hygiene, Faculty of Vet. Med., Assiut University

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Orcid

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First Name

NAGAH

Last Name

M. SAAD

MiddleName

-

Affiliation

Dept. of Food Hygiene, Faculty of Vet. Med., Assiut University

Email

-

City

-

Orcid

-

First Name

RANIA

Last Name

M. EWIDA

MiddleName

-

Affiliation

Dept. of Food Hygiene, Faculty of Vet. Med., Assiut University

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-

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-

Orcid

-

Volume

55

Article Issue

121

Related Issue

24383

Issue Date

2009-04-01

Receive Date

2009-03-19

Publish Date

2009-04-01

Page Start

1

Page End

14

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_174623.html

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https://avmj.journals.ekb.eg/service?article_code=174623

Order

16

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

EFFECT OF NISIN AND CINNAMON ON THE GROWTH AND SURVIVAL OF ENTEROTOXIGENIC METHICILLIN-RESISTANT STAPH. AUREUS IN STERILE MILK AND ICE CREAM

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Article

Created At

23 Jan 2023