The occurrence and identification of Salmonella spp. using a multiplex PCR (m-PCR) in chickens, as well as histopathological changes in experimentally infected chickens and antibiotic sensitivity test were studied. 200 samples (intestine, liver) were collected from freshly dead bird for bacteriological examination. 28 Salmonella isolates were found in chicken samples with overall percentage 14%, by conventional culturing and biochemical reactions. Seven representing isolates were confirmed by a multiplex-PCR (m-PCR) using the three primers pair (ST11-F, ST15-R), (S1-F, S4-R) and Fli15-F, Fli15-R for identification of the most frequent Salmonella enterica serovars. Our results revealed that isolation rate of serovar Enteritidis was 57.14% (16/28) and 42.86% (12/28) were identified as serovarTyphimurium. Histopathologically of the experimentally infected chicken showed thickening of the alveolar wall, blood vessel wall and edema in the lung. Moreover, mononuclear cell infiltration in cardiac muscles and hepatic edema with heterophil cells infiltration in infected liver was also seen. The current study demonstrated that all of 28 Salmonella strains were susceptible to lincospectin, chloramphenicol, erythromycin, ciprofloxacin, doxycyclin, spectinomycin, colistin but were resistant to ampicillin, amoxicillin/clavulanic acid and gentamycin.