Serological study was carried out on the chlamydial related infections in cattle at Assiut Governorate. Eighty five cows in different localities were examined (sixty cows with reproductive disorders in four farms as tested group and twenty five clinically healthy cows in three different farms as a control group). The diseased tested cows were divided into three subgroups; the first tested subgroup (20 cows) with age 3-5 years; the second tested subgroup (25 cows) with age 5-8 years and the third tested subgroup (15 cows) with age more than eight years. Blood samples were taken from each animal with different ages (three years to more than eight years). Samples were examined for both IgG and IgM Chlamydia antibodies with an indirect ELISA. Twenty-one cows of the total examined cows (85) were serologically positive for both IgG and IgM Chlamydia antibodies with a percentage 24.7% (33.3% of the diseased tested cows and 4% of the clinically healthy cows). The serological investigation referred that the infection was higher in diseased tested cows at age 3-5 years; where 35% of these tested cows were serologically positive for both IgG and IgM Chlamydia antibodies. Also seropositivity was 32% and 33.3% of the diseased tested cows in the second (5-8 years) and the third (>8 years) tested subgroups, respectively. It can be concluded that the enzyme immunoassay (ELISA) was easily to perform and can used as rapid screening test for detection of recent, latent chlamydial infections and apparently healthy cows.