The present study was designed to investigate PCR methods for identification and authentication for different kinds of meat based on species specific PCR. Cattle, sheep, goat, donkey, dog and pig meat were used separately and in meat mixture. The effect of thermal treatment of the sample on PCR technique's ability to identify species was studied through the analysis of microwaved meat mixture. Six sets of species specific primers were used to amplify mitochondrial DNA (mt DNA) specific for cattle, sheep, goat, donkey, dog and pig independently. The PCR conditions were optimized on meat of each species separately. The PCR produced a single band of expected size at 271, 225, 157, 439, 322 and 212 bp in the above mentioned animal meat respectively. The results obtained in this study demonstrate the suitability of PCR analysis to identify meat of different animal species with the use of species specific primers. Moreover, the technique could be used to identify the species included in animal feed or in meat products submitted to intense heat treatment as it is rapid, reliable, accurate and easy to perform.