The present study aimed to describe a technique for laparoscopic ovariectomy in dogs, and compare the blood biochemical changes, the surgical time, complications, and postoperative activity of dogs undergoing laparoscopic ovariectomy to those undergoing traditional open ovariectomy. Intact breed female dogs (n=17) were randomized. Ventral median celiotomy was performed for traditional open ovariectomy. A 2-midline portal technique using a laparoscope port and an instrument portal was used for laparoscopic ovariectomy. Blood samples were collected before and at the 1^th, 2^th, 3^th days after laparoscopic ovariectomy and traditional open ovariectomy. White blood cells count and biochemical analysis as blood Suger and Creatine Phosphokinase (CpK) were performed. There is a significant different in the value of Creatine Phosphokinase (CpK) between before (324 ± 62) and after (1576 ± 107) in the traditional open ovariectomy but no significant different in the laparoscopic ovariectomy. No major complications occurred and surgical time for laparoscopic ovariectomy was significantly longer than for traditional open ovariectomy (P < 0.05). Dogs regeurate activity in the laparoscopic ovariectomy after two hours but in traditional open ovariectomy regeurate after two days. Both procedures were performed with reasonable surgical times and without major complication. Blood biochemical changes and postoperative activity was better in laparoscopic ovariectomy