Infectious bronchitis virus (IBV) causes respiratory disease in chickens all over the world. (IBV) has many serotypes that do not confer cross protection against each other. The current study was designed to know which IBV types were circulating in Syrian broiler chickens. Tracheal swabs from 9 broiler flocks at the acute phase of respiratory disease were collected. The swabs were subjected to RNA extraction and tested by reverse transcription PCR (RT-PCR). Specific-nested PCR were performed on RT-PCR products to detect and differentiate strains of Massachusetts, (B/793) 4/91, and D274 types. The nucleic acid of IBV was detected in 5 out 9 (55.6%) broiler flocks by RT-PCR. Specific-nested PCR revealed that 44.45, 33.35, and 11.1 % of these flocks had D274, Massachusetts, and  4/91, respectively. In 33.35 % of tested flocks, 2 types of IBV were detected. However, because the primers used in this study were designed specifically for 3 types of IBV, other types might have been present but not detected. Future work should include the isolation and molecular characterization of IBV in the region to adopt a suitable vaccination program using the common field serotypes as vaccines to protect against IBV-caused disease.