This study is concerned with bacterial causes of subclinical mastitis in baladi cows and buffaloes. A total of 94-baladi cow and 80-buffaloes were examined. All animals rear in a smallholder private cases and hand milked. Screening tests of the milk samples using both field tests (California Mastitis Test and Modified Whiteside Test), revealed that 26(27.74%) & 30(31.9%) baladi cows and 13(16.3%) & 14(17.5%) of buffaloes showed positive results by both tests, respectively. These positive milk samples were examined bacteriologically on general and specific enriched media. The isolated bacteria were 58 isolates from baladi cows and 27 isolates from buffaloes. These isolates resembled two categories: contagious bacteria 14 (24.14%) & 11 (40.74%) and environmental bacteria 44(75.86%) & 16(59.26%) in baladi cows and buffaloes, respectively. The single infection resembled 19.23 and 15.38%, while mixed infection was 80.77 and 84.62% in baladi cows and buffaloes,   respectively. The isolated contagious strains were Staph. aureus and Strept. dysgalactiae 6(10.35%) for both species and Strept. agalactiae 2(3.45%) in baladi cows. In buffaloes, Staph. aureus were 11 isolates (40.74%). Concerning to environmental bacteria in baladi cows E. coli were 12(20.69%); Staph. saprophyticus 10(17.24%);Staph. intermedius 9(15.52%);Strept. Pyogenes and Strept. uberis 3(5.17%) for each, whileStaph. epidermidis and Klebsiella pneumoniae 2(3.45%) for both  and Enterobacter aerogenes was isolated from a single milk sample (1.72%). In buffaloes, E. coliand Staph. intermedius resembled 3(11.1%) for both, Staph. saprophyticus 5(18.52%), Staph. epidermidis 2(7.41%); Strept. uberis andCitrobacter  diversus 1(3.7%)for each. Antimicrobial susceptibility testing revealed that all isolated strains were sensitive to Ciprofloxacin and Ofloxacin with percentage 100% followed by Gentamycin 97.6%, Kanamycin 90.5% and Doxycycline 85.7%. All tested bacterial isolates showed resistance to Cloxacillin, Ampicilin, Amoxicillin and  Cefotaxime. Treatment of some subclinical cases carried on three groups (each of three baladi cows and one buffalo), the first and second group treated with intramammary infusion of Gentamicin and kanamycin, respectively and the third group subjected for intramammary treatment with 10% Egyptian fennel honey solution. All milk samples in the 1^st group were negative for CMT&MWST and bacterial culture post treatment, while in 2^nd group two out of four milk samples at 7^th &10^th days post treatment were positive for CMT&MWST and bacterial culture. The third group all milk samples post treatment were positive for CMT & MWST and negative for bacterial culture. It can be concluded that high prevalence of subclinical mastitis caused by Staphylococcus spp. and E. coli, so strict hygienic measures and effective control for pathogens should be applied. Program of teat dipping and intramammary antibiotic treatment at drying off period is thus recommended. In addition to, enhancing the awareness between dairy farmers is the main point for control of subclinical mastitis in dairy animals.