The present study was aimed to investigate the possible effects of epidermal growth factor (EGF) and insulin-like growth factor 1 (IGF-1) on nuclear maturation, fertilization and embryo development in vitro of buffalo cumulus oocyte complexes (COC's). Oocytes were matured in vitro for 24 h in a defined SOF media with no supplements (control) or in SOF media supplemented with EGF (5, 10 and 20 ng/ml), IGF-1 (25, 50, 100 ng/ml) or with  a combination of EGF and IGF-1. After 24 h of in vitromaturation, oocytes were inseminated with sperm prepared in S-TALP medium supplemented with 10 μg/ml heparin. Embryos were evaluated for cleavage and development to the morula and blastocyst stages. The current results revealed that, addition of a combination of 5 ng/ml EGF plus 50 ng/ml IGF-1 to the maturation medium significantly increased (P<0.01) the in vitro maturation, fertilization rate and embryo development to the morula and blastocyst stages (83.48± 2.27, 62.00±3.19, 29.12±4.13 and 22.87±2.26%, respectively) as compared with the control (61.93±4.09, 32.75±2.77, 7.25±1.45 and 2.80±1.42%, respectively). In conclusion, the current results inferred that EGF and IGF-1 had a positive effect on buffalo ooctes maturation, fertilization and subsequent embryo development in a dose dependent trend. Supplementation of maturation medium with a combination of 5 ng/ml EGF and 50 ng/ml IGF-1 enhanced the in vitro maturation, fertilization and embryo development.