176876

APPLICATION OF ELECTEROPHORESIS AND IMMUNOBLOT IN DIAGNOSIS OF PSEUDOMONAS AERUGINOSA ISOLATED FROM RESPIRATORY SYSTEM OF CAMELS

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Last updated: 05 Jan 2025

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Abstract

This Study was carried out on 70 slaughtered camels. 210 samples (70 each of tracheal swabs, lungs and lymph nodes) for isolation of Pseudomonas aeruginosa. Obtained results declared that Pseudomonas aeruginosa could be detected in 3 (4.28%), 6 (4.28%)and 3 (4.28%) of the examined samples of tracheal swabs, lungs and lymph nodes respectively. The in vitro antibiotic sensitivity tests for the isolated bacteria were discussed. The results showed that Pseudomonas aeruginosa was sensitive to Gentamycin and Chloramephenicol, while this organism was resistant to Amikacin, Flucloxacillin, Penicillin, Neomycin and Ampicillin. SDS- PAGE demonstrated that the protein fractions of Pseudomonas aeruginosa ranged between 62.123 KDa and 18.8 KDa. Immunoblotting analysis of the OMPs of Pseudomonas aeruginosa demonstrated that the monoclonal antibody specific to Pseudomonas aeruginosa OMPs reacted with protein band at molecular weight of 28.017 KDa (more immunogenic band) while faint bands were revealed at 61.203 KDa, 46.148 KDa and 8.1954 KDa.

DOI

10.21608/avmj.2011.176876

Keywords

Key words: Camels, Respiratory System, Pseudomonas aeruginosa

Authors

First Name

EMAN

Last Name

M. SHARAF

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Affiliation

Dept. of Bacteriology, Animal Health Research Institute, Shebin El-Koom.

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First Name

HODA

Last Name

M. ABD EL-MONEM

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Affiliation

Dept. of Biotechnology, Animal Health Research Institute, Dokki, Giza

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Volume

57

Article Issue

130

Related Issue

24338

Issue Date

2011-07-01

Receive Date

2011-06-12

Publish Date

2011-07-01

Page Start

1

Page End

10

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_176876.html

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https://avmj.journals.ekb.eg/service?article_code=176876

Order

19

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

APPLICATION OF ELECTEROPHORESIS AND IMMUNOBLOT IN DIAGNOSIS OF PSEUDOMONAS AERUGINOSA ISOLATED FROM RESPIRATORY SYSTEM OF CAMELS

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Article

Created At

23 Jan 2023